Blood Bank Case Study

------------------------------------------------- Analytical Toxicology Background In analytical toxicology especially to analyze medicines, several(prenominal) methods can be used. The methods can be categorized swear out on their principle of assay which are chemical, spectrometric, chromatographic, electrophoretic and immunochemical assays. each principle of analysis consists of any(prenominal) proficiencys that can be used. However, immunochemical assay is normally used in drug blanket overdue to sensitivity, specificity and low bell than other methods. The half-life of drug excessively used in toxicology analysis to helps in alterative observe and accessing drug abuse. Introduction Immunoassays are bioanalytical method in which the quantitation of analyte depends on the reply of antigen (analyte) and antibody. (Ibrahim A. Darwish , 2006). Immunoassay principle is based on competitive cover charge which specific antibody is used to descry the presence of anal yte or its metabolite in the sample tested. This method consists of some techniques which include: I. Radioimmunoassay (RIA) II. Enzyme immunoassay (EIA) III. Enzyme figure immunoassay technique (EMIT) IV. Enyzme linked immunoasorbent assay (ELISA) V. Fluorescence polarization immunoassay (FPIA) VI. Cloned enzyme donor immunoassay (CEDIA) I.

Radioimmunoassay (RIA) This technique is no longer commonly used due to run through disposal issues where high cost of handling and disposal cost. The analyte in the sample is detected victimization radioisotope. In RIA, sample is complex with specific antibody to the tar ask analy! te and tagged analyte with radioactive iodine. Analyte in the sample compete with labeled analyte for antibody. da Gamma snack bar is used to measure the labeled analyte bind with antibody per slender (CPM). II. Enzyme immunoassay (EIA) EIA is similar to RIA but instead of measuring radiation syndrome this technique measure enzyme activity. It consist of signal detection step which the conduct is measured optically by fluorescence...If you want to get a full essay, order it on our website: OrderCustomPaper.com

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